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N-glycans analysis workflow at NFML
N-glycans analysis workflow at NFML

Glycosylation (the attachment of carbohydrates to macromolecules) plays a key role in modulating the function of various biomolecules. This has an effect on how these molecules execute their respective functions. In addition, food comprises of both free carbohydrate and glycoconjugates. Therefore, unravelling the type, structure, and quantity of glycan moieties as well as free carbohydrates in major biological systems has a huge significance in nutrition, disease diagnosis, monitoring & treatment. NFML employs both LC MS/MS and glycoinformatic approaches to identify, characterize & quantify glycans in various bio samples. Our primary focal point for this is the discovery of both prognostic and diagnostic biomarkers. In addition, we have been endeavoring to profile and characterize glycans in various biomaterials yielding vital biological information on how glycosylation affects cellular function across different systems and organisms. Our key collaborator in this is AGRS.

Research output:

  1. In-depth characterization of non-human sialic acid (Neu5Gc) in human serum using label-free ZIC-HILIC/MRM-MS. Analytical and Bioanalytical Chemistry 2021, 413, 5227-5237.
  2. Isomer-Specific Monitoring of Sialylated N-Glycans Reveals Association of α2,3-Linked Sialic Acid Epitope with Behcet’s Disease. Frontiers in Molecular Biosciences 2021, 1120.
  3. Detection of Aberrant Glycosylation of Serum Haptoglobin for Gastric Cancer Diagnosis Using a Middle-Up-Down Glycoproteome Platform. Journal of Personalized medicine 2021, 11(6), 575.
  4. Spatial and temporal diversity of glycome expression in mammalian brain. Proceedings of the National Academy of Sciences, 2020, 117.46: 28743-28753.
  5. Discovery of N-glycan Biomarkers for the Canine Osteoarthritis. Life (Basel) 2020, 10(9)
  6. Glycosylation of serum haptoglobin as a marker of gastric cancer: an overview for clinicians. Expert Review of Proteomics 2020, 17(2):109-117.
  7. Glycomic profiling of targeted serum haptoglobin for gastric cancer using nano LC/MS and LC/MS/MS. Molecular Biosystems 2016, 12(12):3611-3621.
  8. Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans. Disease Markers, pp. 1–9.
  9. Designation of fingerprint glycopeptides for targeted glycoproteomic analysis of serum haptoglobin: insights into gastric cancer biomarker discovery. Analytical and bioanalytical chemistry 2018, 410(6):1617-1629.
  10. Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer. Oncotarget 2017, 8(7):11094-11104.
  11. Integrated GlycoProteome Analyzer (I-GPA) for Automated Identification and Quantitation of Site-Specific N-Glycosylation. Scientific  Reports 2016, 6:21175.
  12. Analytical detection and characterization of biopharmaceutical glycosylation by MS. Bioanalysis 2016, 8(7):711-727.
  13. Glycosylated proteins preserved over millennia: N-glycan analysis of Tyrolean Iceman, Scythian Princess and Warrior. Scientific Reports 2014, 4:4963.
  14. Serum glycan signatures of gastric cancer. Cancer Prevention Research (Phila) 2014, 7(2):226-235.
  15. Mass spectrometric screening of ovarian cancer with serum glycans. Disease Markers 2014, 2014:634289/634289. 
  16. Differentiation of cancer cell origin and molecular subtype by plasma membrane N-glycan profiling. Journal of Proteome Research 2014, 13, 2, 961–968.

  17. Characterization of novel O-glycans isolated from tear and saliva of ocular rosacea patients.Journal of Proteome Research 2013, 12(3):1090–1100.

  18. The Fifth ACGG-DB Meeting Report: Towards an International Glycan Structure Repository. Glycobiology 2013, 23(12):1422–1424.
  19. The Year of Glycosciences. Glycobiology 2013, 23(2):143–146.
  20. Glycomic analysis of tear and saliva in ocular rosacea patients: the search for a biomarker. Ocular Surface 2012, 10(3):184-192. 
  21. Extensive determination of glycan heterogeneity reveals an unusual abundance of high mannose glycans in enriched plasma membranes of human embryonic stem cells. Molecular & Cellular Proteomics 2012, 11(4):1–13.
  22. Evolutionary glycomics: characterization of milk oligosaccharides in primates. Journal of Proteome Research 2011, 10(4):1548–1557.
  23. High-mannose glycans are elevated during breast cancer progression. Molecular & Cellular Proteomics 2011, 10(1):1–9.
  24. Glycomic approach for potential biomarkers on prostate cancer: profiling of N-linked glycans in human sera and pRNS cell lines. Disease Markers 2008, 25(4-5):243-258.